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KMID : 0861020160310040101
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Korea Journal of Herbology
2016 Volume.31 No. 4 p.101 ~ p.109
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Neuroprotective effects of herbal mixture HT070 on global cerebral ischemia in rats
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Song Jung-Bin
Kim Ho-Cheol
Lee Dong-Hun
Kim Young-Sik
Lee Hyun-Jeong
Lee Seung-Gyeong
Kim Dong-Kuk
Kang Shin-Ho
Shin Yong-Kook
Choi Ho-Young
Kim Ho-Cheol
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Abstract
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Objectives : HT070 is a mixture of herbal extracts from root of Scutellaria baicalensis and stem bark of Eleutherococcus senticosus, which have long been used for stroke therapy in traditional Korean Medicine. The purpose of this study was to investigate the neuroprotective effects of HT070 on global cerebral ischemia and its potential mechanisms.
Methods : Transient global cerebral ischemia was produced by 10 min of four-vessel occlusion (4-VO) in male Wistar rats. HT070 was administered orally at a dosage of 200 mg/kg twice at 0 and 90 min after reperfusion. Hippocampal neuronal damage was measured 7 days after reperfusion. To explore the potential mechanisms, we used hydrogen peroxide (H2O2)-induced rat pheochromocytoma (PC12) cells as an in vitro model. PC12 cells were pretreated with HT070 for 1 h and then exposed to 100 μM H2O2 for 6 h in the presence of HT070. Cell viability was measured by MTT assay and the mRNA expression of Bax, Bcl-2, iNOS and COX-2 were measured by quantitative RT-PCR.
Results : Oral administration of HT070 at a dose of 200 mg/kg significantly reduced neuronal death in the hippocampal CA1 region by 13.4% as compared to the vehicle-treated group. HT070 increased cell viability, reversed the down-regulated Bcl-2 mRNA level, and suppressed the up-regulated mRNA expressions of Bax, iNOS, and COX-2 in H2O2-treated PC12 cells.
Conclusions : HT070 protects against delayed neuronal death after global cerebral ischemia and its neuroprotection properties might be attributed to the inhibition of mitochondrial apoptosis and ROS-generating enzymes.
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KEYWORD
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HT070, Scutellaria baicalensis, Eleutherococcus senticosus, global cerebral ischemia, four-vessel occlusion, neuroprotection, oxidative stress, hydrogen peroxide
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